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Disease Modifying Treatment for Duchenne Muscular Dystrophy (DMD) with a safe Neu-REFIX from Japan; Clinical Study report in IBRO Neuro Science (the official journal of the International Brain Research Organization). Click Here
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Neu REFIX is one of the brands in the Nichi Glucan range of products; specifically, this is a produce of N-163 strain of Black Yeast Aureobasidium Pullulans, comes in a box of 20 sachet (each 8.0 gram of gel; 48mg Beta 1,3-1,6 glucan/Sachet)

 

In pre-clinical studies*, in comparative studies in healthy volunteers, and in patients with Covid-19, the N-163 strain produce has been proven to have potent anti-inflammatory and anti-fibrotic properties. Based on such advantageous outcome, a clinical study in young boys with Duchenne Muscular Dystrophy (DMD) has been completed; a longer duration study under planning, while additional validations in pre-clinical MDX mice models under progress.



 

Publications:


Raghavan K, Sivakumar T, Ichiyama K, Yamamoto N, Balamurugan M, Dedeepiya VD, Senthilkumar R, Preethy S, Abraham S. Efficacy of N-163 beta-glucan in beneficially improving biomarkers of relevance to muscle function in patients with muscular dystrophies in a pilot clinical study. Acta Myologica 2023. 2023 Dec 20; doi: 10.36185/2532-1900-312


Preethy S, Aoki Y, Minegishi K, Iwasaki M, Senthilkumar R, Abraham S. Resolution of fibrosis in mdx dystrophic mouse after oral consumption of N-163 strain of Aureobasidium pullulans produced β-glucan. Sci Rep 13, 17008 (2023). doi: 10.1038/s41598-023-44330-0


Senthilkumar Preethy, Naoki Yamamoto, Kottoorathu Mammen Cherian, Rajasekaran Premsekar, Gary A Levy, Rajappa Senthilkumar, Samuel JK Abraham. Reduction in myocardial fibrosis in MDX mice on oral consumption of Aureobasidium Pullulans produced Neu REFIX Beta-glucans; holds potential as an adjuvant in managing post-transplantation organ fibrosis. biorxiv: 10.1101/2023.09.25.559276v1. doi: 10.1101/2023.09.25.559276 (In pre-print)


Raghavan K, Dedeepiya VD, Srinivasan S, Pushkala S, Subramanian S, Ikewaki N, Iwasaki M, Senthilkumar R, Preethy S, Abraham S. Beneficial immune-modulatory effects of the N-163 strain of Aureobasidium pullulans-produced 1,3-1,6 Beta glucans in Duchenne muscular dystrophy: Results of an open-label, prospective, exploratory case-control clinical study. IBRO Neuroscience reports 2023; 15: 90-99. doi: 10.1016/j.ibneur.2023.06.007.


Ikewaki N, Sonoda T, Kurosawa G, Iwasaki M, Dedeepiya VD, Senthilkumar R, Preethy S, Abraham S . Beta 1,3-1,6 glucans produced by two novel strains of Aureobasidium pullulans exert immune and metabolic beneficial effects in healthy middle-aged Japanese men: Results of an exploratory randomized control study. J Aging Res & Lifestyle 2023;12:61-71. doi: 10.14283/jarlife.2023.11.


Kadalraja Raghavan, Vidyasagar Devaprasad Dedeepiya, Subramaniam Srinivasan, Subramanian Pushkala, Sudhakar S. Bharatidasan, Nobunao Ikewaki, Masaru Iwasaki, Rajappa Senthilkumar, Senthilkumar Preethy, Samuel J.K. Abraham. Beneficial immune-modulatory effects of the N-163 strain of Aureobasidium pullulans-produced 1,3-1,6 Beta glucans in Duchenne muscular dystrophy: Results of an open-label, prospective, exploratory case-control clinical study. IBRO Neuroscience Reports 15 (2023) 90-99; doi : 10.1016/j.ibneur.2023.06.007.


Senthilkumar Preethy, Shuji Sakamoto, Takuma Higuchi, Koji Ichiyama, Naoki Yamamoto, Nobunao Ikewaki, Masaru Iwasaki, Vidyasagar Devaprasad Dedeepiya, Subramaniam Srinivasan, Mathaiyan Rajmohan, Rajappa Senthilkumar, Samuel JK Abraham. Enhanced muscle regeneration in mdx mice, Duchenne muscular dystrophy animal model, proven by CD44 & MYH3 expression, on oral feeding of N-163 strain of Aureobasidium Pullulans produced B-Glucan. biorxiv 2023.06.06.543858v1 doi: 10.1101/2023.06.06.543858 (In pre-print)


Vidyasagar Devaprasad Dedeepiya, Chockanathan Vetrievel, Nobunao Ikewaki, Koji Ichiyama, Naoki Yamamoto, Hiroto Kawashima, Sudhakar S Bharatidasan, Subramaniam Srinivasan, Rajappa Senthilkumar, Senthilkumar Preethy, Samuel JK Abraham. Improvement in Expanded Disability Status Scale (EDSS) and anti-inflammatory parameters in patients with multiple sclerosis following oral consumption of N-163 strain of Aureobasidium pullulans produced beta glucan in a pilot clinical study. medRxiv 2023.05.14.23289953v2 doi: 10.1101/2023.05.14.23289953 (In pre-print)


Kadalraja Raghavan, Thanasekar Sivakumar, Sudhakar S Bharatidasan, Subramaniam Srinivasan, Vidyasagar Devaprasad Dedeepiya, Nobunao Ikewaki, Rajappa Senthilkumar, Senthilkumar Preethy, Samuel JK Abraham. Efficacy of N-163 strain of Aureobasidium pullulans-produced beta-glucan in improving muscle strength and function in patients with Duchenne muscular dystrophy; Results of a 6-month non-randomised open-label linear clinical trial. medRxiv 2023.04.29.23289260v1; doi: 10.1101/2023.04.29.23289260 (In pre-print)


Raghavan K, Dedeepiya VD, Yamamoto N, Ikewaki N, Iwasaki M, Dinassing A, Senthilkumar R, Preethy S, Abraham SJK. Randomised trial of Aureobasidium pullulans-produced beta 1,3-1,6-glucans in patients with Duchenne muscular dystrophy: Favourable changes in gut microbiota and clinical outcomes indicating their potential in epigenetic manipulation. medRxiv 2022.12.09.22283273; doi: 10.1101/2022.12.09.22283273 (In pre-print)


Raghavan K, Dedeepiya VD, Srinivasan S, Pushkala S, Subramanian S, Ikewaki N, Iwasaki M, Senthilkumar R, Preethy S, Abraham S. Disease-modifying immune-modulatory effects of the N-163 strain of Aureobasidium pullulans-produced 1,3-1,6 Beta glucans in young boys with Duchenne muscular dystrophy: Results of an open-label, prospective, randomized, comparative clinical study medRxiv 10.1101/2021.12.13.21267706v1; doi: 10.1101/2021.12.13.21267706 (In pre-print)

 

 

Conference Presentations:


3~7, October 2023: Samuel JK Abraham, Gary A Levy, Naoki Yamamoto, Kottoorathu Mammen Cherian, Rajasekaran Premsekar, Rajappa Senthilkumar, Senthilkumar Preethy. Amelioration of myocardial fibrosis in mdx mice of Duchenne muscular dystrophy (DMD) on oral consumption of Aureobasidium Pullulans produced Neu REFIX Beta glucans. World Muscle Society, WMS2023, 3~7, October 2023, Charleston, USA.


Samuel JK Abraham, Kadalraja Raghavan, Thanasekar Sivakumar, Sudhakar S Bharathidasan, Subramaniam Srinivasan, Vidyasagar Devaprasad Dedeepiya, Nobunao Ikewaki, Masaru Iwasaki, Rajappa Senthilkumar, Senthilkumar Preethy. Efficacy of a safe ß-glucan in improving muscle function in Duchenne Muscular Dystrophy; results of a 6-month nonrandomized open-label extension trial. MDA Conference 2023.


Samuel JK Abraham, Shuji Sakamoto, Takuma Higuchi, Subramaniam Srinivasan, Vidyasagar Devaprasad Dedeepiya, Nobunao Ikewaki, Masaru Iwasaki, Mathaiyan Rajmohan, Rajappa Senthilkumar, Senthilkumar Preethy. Enhanced muscle regeneration in MDX Duchenne muscular dystrophy-mice, proven by CD44 & MYH3, on consumption of A.Pullulans produced Neu REFIX ß-Glucan. MDA Conference 2023.


 

* Links to publications are posted for educational purposes only.


Neu REFIX is a food supplement, not a drug or substitute to any drug or remedy to illness. No therapeutic claims; those who want to discuss about the clinical studies reported, may consult a physician.


Nichi Glucan range of products available in the brands, viz., Nichi Glucan, Nichi Glucan REFIX, Neu-REFIX, Nichi BRITE, and Nichi GLOW are free from any of the following notified common allergens listed as per the Consumer Affairs Agency, Government of Japan. (Japanese)

Shrimp, crab, wheat, buckwheat, egg, milk, peanut (peanuts), Almond, abalone, squid, salmon roe, orange, cashew nut, kiwi fruit, beef, walnut, sesame, salmon, mackerel, soybean, chicken, banana, pork, matsutake mushroom, peach, yam, apple, gelatin.

 

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